Bismark

Bismark Processing Report

SRR2034992_GSM1677166_YoungBeta_BIS-Seq_Mus_musculus_Bisulfite-Seq_1_val_1.fq.gz and SRR2034992_GSM1677166_YoungBeta_BIS-Seq_Mus_musculus_Bisulfite-Seq_2_val_2.fq.gz

Data processed at 19:06:37 on 2022-12-14


Alignment Stats

Sequence pairs analysed in total 168603085
Paired-end alignments with a unique best hit 84901243
Pairs without alignments under any condition 62721003
Pairs that did not map uniquely 20980839
Genomic sequence context not extractable (edges of chromosomes) 2

Cytosine Methylation

Total C's analysed 2761757972
Methylated C's in CpG context 183818975
Methylated C's in CHG context 69991458
Methylated C's in CHH context 250674292
Methylated C's in Unknown context 56576
Unmethylated C's in CpG context 63916187
Unmethylated C's in CHG context 647127477
Unmethylated C's in CHH context 1546229583
Unmethylated C's in Unknown context 287613
Percentage methylation (CpG context) 74.2%
Percentage methylation (CHG context) 9.8%
Percentage methylation (CHH context) 14.0%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 45574886 original top strand
CTOT 0 complementary to original top strand
CTOB 0 complementary to original bottom strand
OB 39326355 original bottom strand

Deduplication

Alignments analysed 84901241
Unique alignments 41762632
Duplicates removed 43138609
Duplicated alignments were found at 17923915 different positions

Cytosine Methylation after Extraction

Total C's analysed 674912293
Methylated C's in CpG context 29710158
Methylated C's in CHG context 17150336
Methylated C's in CHH context 58901119
Unmethylated C's in CpG context 15083916
Unmethylated C's in CHG context 166544459
Unmethylated C's in CHH context 387522305
Percentage methylation (CpG context) 66.3%
Percentage methylation (CHG context) 9.3%
Percentage methylation (CHH context) 13.2%

M-Bias Plot