FastQCFastQC Report
Tue 2 May 2023
SRR2034992_GSM1677166_YoungBeta_BIS-Seq_Mus_musculus_Bisulfite-Seq_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2034992_GSM1677166_YoungBeta_BIS-Seq_Mus_musculus_Bisulfite-Seq_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences185532031
Total Bases18.5 Gbp
Sequences flagged as poor quality0
Sequence length100
%GC30

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGNCATTCCTGCTGAACCGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGT10126280.545796860273685Illumina Single End PCR Primer 1 (100% over 31bp)
CGNCATTCCTGCTGAACCGCTCTTCCGAGATCGGAAGAGCGTCGTGTAGG3265300.17599656417279236Illumina Paired End Adapter 2 (96% over 28bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)