Bismark

Bismark Processing Report

SRR2034988_GSM1677165_OldBeta_BIS-Seq_Mus_musculus_Bisulfite-Seq_1_val_1.fq.gz and SRR2034988_GSM1677165_OldBeta_BIS-Seq_Mus_musculus_Bisulfite-Seq_2_val_2.fq.gz

Data processed at 19:06:36 on 2022-12-14


Alignment Stats

Sequence pairs analysed in total 89547646
Paired-end alignments with a unique best hit 45492827
Pairs without alignments under any condition 36115043
Pairs that did not map uniquely 7939776
Genomic sequence context not extractable (edges of chromosomes) 3

Cytosine Methylation

Total C's analysed 1512440586
Methylated C's in CpG context 79809662
Methylated C's in CHG context 31641705
Methylated C's in CHH context 102167121
Methylated C's in Unknown context 34150
Unmethylated C's in CpG context 29770477
Unmethylated C's in CHG context 383126027
Unmethylated C's in CHH context 885925594
Unmethylated C's in Unknown context 154420
Percentage methylation (CpG context) 72.8%
Percentage methylation (CHG context) 7.6%
Percentage methylation (CHH context) 10.3%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 23263096 original top strand
CTOT 0 complementary to original top strand
CTOB 0 complementary to original bottom strand
OB 22229728 original bottom strand

Deduplication

Alignments analysed 45492824
Unique alignments 37406198
Duplicates removed 8086626
Duplicated alignments were found at 4843525 different positions

Cytosine Methylation after Extraction

Total C's analysed 624280580
Methylated C's in CpG context 28254725
Methylated C's in CHG context 12972798
Methylated C's in CHH context 41105565
Unmethylated C's in CpG context 11856634
Unmethylated C's in CHG context 161100228
Unmethylated C's in CHH context 368990630
Percentage methylation (CpG context) 70.4%
Percentage methylation (CHG context) 7.5%
Percentage methylation (CHH context) 10.0%

M-Bias Plot