FastQCFastQC Report
Tue 2 May 2023
SRR2034988_GSM1677165_OldBeta_BIS-Seq_Mus_musculus_Bisulfite-Seq_2_val_2.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2034988_GSM1677165_OldBeta_BIS-Seq_Mus_musculus_Bisulfite-Seq_2_val_2.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences89547646
Total Bases7.8 Gbp
Sequences flagged as poor quality0
Sequence length20-100
%GC30

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGCATTCCTGCTGAACCGCTCTTCCG8578040.9579302620640636Illumina Paired End Adapter 2 (100% over 27bp)
CGGCATTCCTGCTGAACCGCTCTTCCGA1503830.16793629617019748Illumina Paired End Adapter 2 (100% over 28bp)
CGGCATTCCTGCTGAACCGC1292160.14429860054612714No Hit
CGGCATTCCTNCTGAACCGCTCTTCCG1111770.12415401740432128Illumina Paired End Adapter 2 (96% over 27bp)
TCGGCATTCCTGCTGAACCGCTCTTCCGA1008520.11262384273060624Illumina Paired End Adapter 2 (100% over 29bp)
CGGCATTCCTGCTGAACCGCT950270.10611892578393407Illumina Paired End Adapter 2 (100% over 21bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)