FastQCFastQC Report
Tue 2 May 2023
SRR2034988_GSM1677165_OldBeta_BIS-Seq_Mus_musculus_Bisulfite-Seq_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2034988_GSM1677165_OldBeta_BIS-Seq_Mus_musculus_Bisulfite-Seq_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences97036309
Total Bases9.7 Gbp
Sequences flagged as poor quality0
Sequence length100
%GC33

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCG9362780.9648738803533841Illumina Paired End PCR Primer 2 (100% over 31bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTATGCCGT6065550.6250804531322394Illumina Paired End PCR Primer 2 (100% over 50bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAG5666970.5840051067894596Illumina Paired End PCR Primer 2 (96% over 33bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTATGCCGTCTT1549240.15965570166111737Illumina Paired End PCR Primer 2 (100% over 50bp)
TCGGAAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCA975600.10053968561396952Illumina Paired End PCR Primer 2 (97% over 34bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)