FastQCFastQC Report
Tue 2 May 2023
SRR14760800_GSM5363743_RNA-seq_Tet1_KO_Replicate_R3_Mus_musculus_RNA-Seq_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR14760800_GSM5363743_RNA-seq_Tet1_KO_Replicate_R3_Mus_musculus_RNA-Seq_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35548711
Total Bases3.4 Gbp
Sequences flagged as poor quality0
Sequence length20-100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCGTTCTCGCCCTCGCTCTCCCTCCACACAAGCCGGAGGGTGCGCGGC678980.19099989307629187No Hit
CCCGAGTGTCCGGGCCCCCCGCCCCACCGG656140.18457490624624898No Hit
CCCGAGTGTCCGGGCCCCCCGCCCCACCGGGGGCCCGCTGGTTCCTCCCG624500.17567444287923686No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCTTG360150.10131169031698505No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)