FastQCFastQC Report
Tue 2 May 2023
SRR14760799_GSM5363742_RNA-seq_Tet1_KO_Replicate_R2_Mus_musculus_RNA-Seq_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR14760799_GSM5363742_RNA-seq_Tet1_KO_Replicate_R2_Mus_musculus_RNA-Seq_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35441745
Total Bases3.4 Gbp
Sequences flagged as poor quality0
Sequence length20-100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCGTTCTCGCCCTCGCTCTCCCTCCACACAAGCCGGAGGGTGCGCGGC700130.1975438850429063No Hit
CCCGAGTGTCCGGGCCCCCCGCCCCACCGG658160.18570191732940913No Hit
CCCGAGTGTCCGGGCCCCCCGCCCCACCGGGGGCCCGCTGGTTCCTCCCG574690.16215059388300435No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCTTG362760.10235387676312212No Hit
CCCGGGACAGGAGCGGGGAGGACACGCGAGGCCAGAAAAGCGTGGCATCG355240.10023208507368923No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)