FastQCFastQC Report
Tue 2 May 2023
SRR14760798_GSM5363741_RNA-seq_Tet1_KO_Replicate_R1_Mus_musculus_RNA-Seq_1_val_1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR14760798_GSM5363741_RNA-seq_Tet1_KO_Replicate_R1_Mus_musculus_RNA-Seq_1_val_1.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32828296
Total Bases3.1 Gbp
Sequences flagged as poor quality0
Sequence length20-100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCGTTCTCGCCCTCGCTCTCCCTCCACACAAGCCGGAGGGTGCGCGGC698170.21267323774587632No Hit
CCCGAGTGTCCGGGCCCCCCGCCCCACCGG631650.1924102304914029No Hit
CCCGAGTGTCCGGGCCCCCCGCCCCACCGGGGGCCCGCTGGTTCCTCCCG510440.15548781453658148No Hit
CCCGGGACAGGAGCGGGGAGGACACGCGAGGCCAGAAAAGCGTGGCATCG367490.1119430627773065No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCTTG347790.10594214210813745No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)