FastQCFastQC Report
Tue 2 May 2023
SRR14760793_GSM5363736_RNA-seq_Tet1_WT_Replicate_R2_Mus_musculus_RNA-Seq_1_val_1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR14760793_GSM5363736_RNA-seq_Tet1_WT_Replicate_R2_Mus_musculus_RNA-Seq_1_val_1.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35894953
Total Bases3.4 Gbp
Sequences flagged as poor quality0
Sequence length20-100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCGTTCTCGCCCTCGCTCTCCCTCCACACAAGCCGGAGGGTGCGCGGC834140.23238364457532512No Hit
CCCGAGTGTCCGGGCCCCCCGCCCCACCGG655320.1825660560135014No Hit
CCCGAGTGTCCGGGCCCCCCGCCCCACCGGGGGCCCGCTGGTTCCTCCCG552110.15381271010439823No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCTTG412310.11486573056663425No Hit
CCCGGGACAGGAGCGGGGAGGACACGCGAGGCCAGAAAAGCGTGGCATCG411620.11467350298522469No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)