FastQCFastQC Report
Tue 2 May 2023
SRR14760793_GSM5363736_RNA-seq_Tet1_WT_Replicate_R2_Mus_musculus_RNA-Seq_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR14760793_GSM5363736_RNA-seq_Tet1_WT_Replicate_R2_Mus_musculus_RNA-Seq_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35942263
Total Bases3.4 Gbp
Sequences flagged as poor quality0
Sequence length20-100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCGTTCTCGCCCTCGCTCTCCCTCCACACAAGCCGGAGGGTGCGCGGC837090.2328985239465862No Hit
CCCGAGTGTCCGGGCCCCCCGCCCCACCGG656710.182712479734512No Hit
CCCGAGTGTCCGGGCCCCCCGCCCCACCGGGGGCCCGCTGGTTCCTCCCG558350.1553463675895978No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCTTG412860.11486755856190801No Hit
CCCGGGACAGGAGCGGGGAGGACACGCGAGGCCAGAAAAGCGTGGCATCG412320.11471731760462607No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)