FastQCFastQC Report
Tue 2 May 2023
SRR14760792_GSM5363735_RNA-seq_Tet1_WT_Replicate_R1_Mus_musculus_RNA-Seq_1_val_1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR14760792_GSM5363735_RNA-seq_Tet1_WT_Replicate_R1_Mus_musculus_RNA-Seq_1_val_1.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36691023
Total Bases3.5 Gbp
Sequences flagged as poor quality0
Sequence length20-100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCGTTCTCGCCCTCGCTCTCCCTCCACACAAGCCGGAGGGTGCGCGGC983220.2679729044349622No Hit
CCCGAGTGTCCGGGCCCCCCGCCCCACCGG650740.17735673382560088No Hit
CCCGAGTGTCCGGGCCCCCCGCCCCACCGGGGGCCCGCTGGTTCCTCCCG501610.1367119145192545No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCTTG456780.12449366702040443No Hit
CCCGGGACAGGAGCGGGGAGGACACGCGAGGCCAGAAAAGCGTGGCATCG402530.10970803403328383No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)