FastQCFastQC Report
Tue 2 May 2023
SRR14760792_GSM5363735_RNA-seq_Tet1_WT_Replicate_R1_Mus_musculus_RNA-Seq_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR14760792_GSM5363735_RNA-seq_Tet1_WT_Replicate_R1_Mus_musculus_RNA-Seq_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36738470
Total Bases3.5 Gbp
Sequences flagged as poor quality0
Sequence length20-100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCGTTCTCGCCCTCGCTCTCCCTCCACACAAGCCGGAGGGTGCGCGGC986750.26858766845761406No Hit
CCCGAGTGTCCGGGCCCCCCGCCCCACCGG652350.1775659138771974No Hit
CCCGAGTGTCCGGGCCCCCCGCCCCACCGGGGGCCCGCTGGTTCCTCCCG506940.13798614912379312No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCTTG457450.12451525607898206No Hit
CCCGGGACAGGAGCGGGGAGGACACGCGAGGCCAGAAAAGCGTGGCATCG403360.10979226952020592No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)