FastQCFastQC Report
Tue 8 Mar 2016
pacbio_srr075104.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamepacbio_srr075104.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48053
Sequences flagged as poor quality0
Sequence length82-6919
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACTC300.00941203131.764149
GCAAGCT1000.004270206759.2938581
GGTACAT250.0060743454.30679300-309
TCCTTGC1350.00620694510.999148190-199