FastQCFastQC Report
Tue 16 Aug 2011
bad_sequence.txt

Summary

[OK] Basic Statistics

Measure Value
Filename bad_sequence.txt
File type Conventional base calls
Encoding Illumina 1.5
Total Sequences 395288
Sequence length 40
%GC 47

[FAIL] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[WARN] Per base sequence content

Per base sequence content

[WARN] Per base GC content

Per base GC content graph

[WARN] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[WARN] Sequence Duplication Levels

Duplication level graph

[WARN] Overrepresented sequences

Sequence Count Percentage Possible Source
AGAGTTTTATCGCTTCCATGACGCAGAAGTTAACACTTTC 2065 0.5224039181558763 No Hit
GATTGGCGTATCCAACCTGCAGAGTTTTATCGCTTCCATG 2047 0.5178502762542754 No Hit
ATTGGCGTATCCAACCTGCAGAGTTTTATCGCTTCCATGA 2014 0.5095019327680071 No Hit
CGATAAAAATGATTGGCGTATCCAACCTGCAGAGTTTTAT 1913 0.4839509420979134 No Hit
GTATCCAACCTGCAGAGTTTTATCGCTTCCATGACGCAGA 1879 0.47534961850600066 No Hit
AAAAATGATTGGCGTATCCAACCTGCAGAGTTTTATCGCT 1846 0.4670012750197325 No Hit
TGATTGGCGTATCCAACCTGCAGAGTTTTATCGCTTCCAT 1841 0.46573637449150995 No Hit
AACCTGCAGAGTTTTATCGCTTCCATGACGCAGAAGTTAA 1836 0.46447147396328753 No Hit
GATAAAAATGATTGGCGTATCCAACCTGCAGAGTTTTATC 1831 0.4632065734350651 No Hit
AAATGATTGGCGTATCCAACCTGCAGAGTTTTATCGCTTC 1779 0.45005160794155147 No Hit
ATGATTGGCGTATCCAACCTGCAGAGTTTTATCGCTTCCA 1779 0.45005160794155147 No Hit
AATGATTGGCGTATCCAACCTGCAGAGTTTTATCGCTTCC 1760 0.4452449859343061 No Hit
AAAATGATTGGCGTATCCAACCTGCAGAGTTTTATCGCTT 1729 0.4374026026593269 No Hit
CGTATCCAACCTGCAGAGTTTTATCGCTTCCATGACGCAG 1713 0.43335492096901496 No Hit
ATCCAACCTGCAGAGTTTTATCGCTTCCATGACGCAGAAG 1708 0.43209002044079253 No Hit
CAGAGTTTTATCGCTTCCATGACGCAGAAGTTAACACTTT 1684 0.42601849790532476 No Hit
TGCAGAGTTTTATCGCTTCCATGACGCAGAAGTTAACACT 1668 0.4219708162150128 No Hit
CAACCTGCAGAGTTTTATCGCTTCCATGACGCAGAAGTTA 1668 0.4219708162150128 No Hit
TATCCAACCTGCAGAGTTTTATCGCTTCCATGACGCAGAA 1630 0.4123575722005221 No Hit
GTCATGGAAGCGATAAAACTCTGCAGGTTGGATACGCCAA 1620 0.40982777114407726 No Hit
AACTTCTGCGTCATGGAAGCGATAAAACTCTGCAGGTTGG 1616 0.4088158507214993 No Hit
GCAGAGTTTTATCGCTTCCATGACGCAGAAGTTAACACTT 1580 0.39970856691829754 No Hit
TGGCGTATCCAACCTGCAGAGTTTTATCGCTTCCATGACG 1569 0.3969257857562082 No Hit
GGCGTATCCAACCTGCAGAGTTTTATCGCTTCCATGACGC 1542 0.39009532290380683 No Hit
ATAAAAATGATTGGCGTATCCAACCTGCAGAGTTTTATCG 1481 0.37466353645949285 No Hit
ACCTGCAGAGTTTTATCGCTTCCATGACGCAGAAGTTAAC 1479 0.37415757624820384 No Hit
ATGGAAGCGATAAAACTCTGCAGGTTGGATACGCCAATCA 1452 0.3673271133958026 No Hit
GATAAAACTCTGCAGGTTGGATACGCCAATCATTTTTATC 1420 0.35923175001517876 No Hit
CGTCATGGAAGCGATAAAACTCTGCAGGTTGGATACGCCA 1412 0.3572079091700229 No Hit
ACTTCTGCGTCATGGAAGCGATAAAACTCTGCAGGTTGGA 1368 0.34607678452166524 No Hit
TAACTTCTGCGTCATGGAAGCGATAAAACTCTGCAGGTTG 1363 0.34481188399344276 No Hit
CATGGAAGCGATAAAACTCTGCAGGTTGGATACGCCAATC 1333 0.337222480824108 No Hit
CGATAAAACTCTGCAGGTTGGATACGCCAATCATTTTTAT 1304 0.32988605776041774 No Hit
TAAAAATGATTGGCGTATCCAACCTGCAGAGTTTTATCGC 1277 0.32305559490801644 No Hit
GCGTATCCAACCTGCAGAGTTTTATCGCTTCCATGACGCA 1262 0.31926089332334906 No Hit
TGCGTCATGGAAGCGATAAAACTCTGCAGGTTGGATACGC 1233 0.3119244702596588 No Hit
GGAAGCGATAAAACTCTGCAGGTTGGATACGCCAATCATT 1182 0.2990224848717897 No Hit
AAGCGATAAAACTCTGCAGGTTGGATACGCCAATCATTTT 1136 0.2873854000121431 No Hit
ACTCTGCAGGTTGGATACGCCAATCATTTTTATCGAAGCG 1133 0.28662645969520956 No Hit
AAACTCTGCAGGTTGGATACGCCAATCATTTTTATCGAAG 1131 0.2861204994839206 No Hit
AAAACTCTGCAGGTTGGATACGCCAATCATTTTTATCGAA 1129 0.2856145392726316 No Hit
AGCGATAAAACTCTGCAGGTTGGATACGCCAATCATTTTT 1113 0.2815668575823197 No Hit
ATAAAACTCTGCAGGTTGGATACGCCAATCATTTTTATCG 1111 0.28106089737103074 No Hit
AACTCTGCAGGTTGGATACGCCAATCATTTTTATCGAAGC 1083 0.273977454412985 No Hit
CTGCAGAGTTTTATCGCTTCCATGACGCAGAAGTTAACAC 1055 0.2668940114549392 No Hit
TTCTGCGTCATGGAAGCGATAAAACTCTGCAGGTTGGATA 947 0.23957216004533402 No Hit
TGGAAGCGATAAAACTCTGCAGGTTGGATACGCCAATCAT 946 0.23931917993968954 No Hit
TAAAACTCTGCAGGTTGGATACGCCAATCATTTTTATCGA 912 0.2307178563477768 No Hit
GAAGCGATAAAACTCTGCAGGTTGGATACGCCAATCATTT 888 0.224646333812309 No Hit
GCGTCATGGAAGCGATAAAACTCTGCAGGTTGGATACGCC 805 0.20364898504381615 No Hit
GCGATAAAACTCTGCAGGTTGGATACGCCAATCATTTTTA 785 0.19858938293092632 No Hit
TTGGCGTATCCAACCTGCAGAGTTTTATCGCTTCCATGAC 784 0.1983364028252818 No Hit
CTTCTGCGTCATGGAAGCGATAAAACTCTGCAGGTTGGAT 762 0.192770840501103 No Hit
TCCAACCTGCAGAGTTTTATCGCTTCCATGACGCAGAAGT 752 0.19024103944465806 No Hit
CCAACCTGCAGAGTTTTATCGCTTCCATGACGCAGAAGTT 744 0.18821719859950212 No Hit
TCATGGAAGCGATAAAACTCTGCAGGTTGGATACGCCAAT 665 0.16823177025358726 No Hit
TCTGCGTCATGGAAGCGATAAAACTCTGCAGGTTGGATAC 627 0.15861852623909656 No Hit
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCT 624 0.1578595859221631 Illumina Paired End PCR Primer 2 (100% over 40bp)
CCTGCAGAGTTTTATCGCTTCCATGACGCAGAAGTTAACA 613 0.15507680476007366 No Hit
CGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGC 599 0.15153508328105078 Illumina Paired End PCR Primer 2 (96% over 25bp)
TCTGCAGGTTGGATACGCCAATCATTTTTATCGAAGCGCG 585 0.1479933618020279 No Hit
CGCTTAAAGCTACCAGTTATATGGCTGGGGGGTTTTTTTT 552 0.13964501831575965 No Hit
CTCTGCAGGTTGGATACGCCAATCATTTTTATCGAAGCGC 532 0.1345854162028698 No Hit
CTGCGTCATGGAAGCGATAAAACTCTGCAGGTTGGATACG 515 0.13028475440691342 No Hit
CTGCAGGTTGGATACGCCAATCATTTTTATCGAAGCGCGC 505 0.12775495335046852 No Hit
GCTTAAAGCTACCAGTTATATGGCTGGGGGGTTTTTTTTG 411 0.10397482341988626 No Hit

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TTTTT 192940 8.590186 21.06293 29
CTGCA 90975 7.7906475 12.251836 10
GCAGA 84910 7.163295 13.539302 23
TGCAG 92470 7.002405 10.671717 11
CCTGC 57235 5.4987235 8.729035 16
GTTTT 108205 5.324498 10.243909 28
CAACC 49005 5.2869425 9.85526 13
ATCGC 58320 4.9942355 8.029807 29
CCAAC 46220 4.9864807 9.408141 12
AAAAA 62285 4.7588468 8.0126295 5
CAGAG 56370 4.7555633 7.148592 20
ACCTG 55315 4.736902 7.919266 15
CGCCA 44035 4.7130895 8.830201 35
GGGGG 63675 4.67525 16.94222 27
GCAGG 55380 4.6350074 17.521912 19
AAAAC 51945 4.452569 8.159592 24
TATCG 64615 4.4271946 8.394971 34
GCTGG 58505 4.3952427 10.37436 18
AACCT 50775 4.382863 7.691214 14
TTATC 70080 4.3444843 7.810299 33
TTTTA 87340 4.332125 7.8541703 28
TTTAT 86645 4.297653 7.9511886 35
CGCTT 54695 4.2042785 6.9374876 31
CTTCC 48210 4.1906962 7.8460555 33
TGGCG 55310 4.1552153 7.5370812 14
ACGCC 38740 4.146363 7.923584 31
TCTGC 52650 4.0470843 8.286615 5
TCGCT 52250 4.0163374 6.828795 23
ATCCA 46415 4.0065107 8.820675 1
GCTTC 50460 3.8787441 6.9236975 32
AGAGT 57395 3.8741086 5.6934085 21
TCCAA 43220 3.7307208 8.166326 2
CGCAG 38285 3.623525 8.489245 22
ACGCA 37480 3.57568 8.295359 21
GAGTT 58985 3.5738122 5.2733626 22
TTCCA 46030 3.5664926 6.9852405 34
ATAAA 51605 3.5391784 10.336252 2
CCATG 40730 3.4879153 7.5872197 29
CAGAA 40865 3.4750571 8.002395 24
TAAAA 50115 3.436991 9.676721 3
AGTTT 62490 3.4257019 4.999059 23
CAGGT 44225 3.3489926 5.670725 24
GACGC 35370 3.3476324 8.1835375 33
TATCC 42050 3.2581146 6.69368 9
AACTC 37735 3.2572594 5.9674845 17
TCCAT 41605 3.2236352 6.9067335 28
GAAGC 38205 3.223103 7.652143 7
ATGGC 41625 3.1521046 10.790563 20
GATAA 45445 3.092006 10.076592 1
AAACT 40050 3.0814974 5.571396 16
CTCTG 39455 3.0328155 5.248931 28
CCCCC 22315 3.0301106 6.8509517 30
TGACG 40010 3.0298069 6.8470154 32
CATGA 39680 3.0288272 6.7769623 30
GGAAG 40220 3.0004814 14.347525 5
GCCAA 31370 2.9927719 6.411429 25
GGCGT 39800 2.9900124 6.854864 5
ACCAG 31330 2.9889555 9.309048 11
GGCGG 35505 2.948021 9.878704 20
ACTCT 37990 2.943538 5.1828785 18
AAGCG 34530 2.9130673 6.3572273 8
GCGTA 38210 2.8934996 6.830749 6
TACGC 33560 2.8739119 5.985593 33
ATGAC 37105 2.8322742 6.810046 31
CGATA 36955 2.8208244 9.8949795 1
GGTTG 46665 2.804949 5.566917 26
AATCA 35955 2.7664232 5.637351 28
GCGAT 35815 2.712135 5.5935216 10
GCGGG 32645 2.7105515 9.132057 20
GTATC 39480 2.7050319 5.954676 8
AGCGA 31360 2.6456356 5.906183 9
GATAC 34020 2.5967922 5.3611007 31
CAATC 29950 2.5852633 5.9689703 27
GGGTT 42825 2.574133 8.646748 25
CGTAT 36955 2.5320277 6.0020866 7
TGCTG 37155 2.5255504 5.032586 10
ATACG 32730 2.4983244 5.163124 32
CCAAT 28765 2.482975 5.6009073 26
GGTTT 45150 2.455502 6.9169445 26
CAGCA 25285 2.4122484 16.940851 17
TTGGC 35225 2.394362 6.1196923 6
CGGGG 28680 2.381333 7.050055 22
CCAGT 27650 2.367809 7.8966165 12
TGGCT 34735 2.3610547 8.305194 21
CTGGC 27565 2.3418174 5.5656714 17
AGAAG 30730 2.3108296 6.6652923 25
GGGGT 34390 2.2846353 8.805303 24
AAAGC 26820 2.2807055 8.617608 2
AGTTA 37050 2.262741 5.220223 14
TATGG 37165 2.251771 8.2647915 19
GGCTG 29890 2.2455144 7.5107794 22
AGCGG 26735 2.2375755 15.021941 10
CGTCA 26040 2.2299364 7.7548785 3
TCAGC 25505 2.1841218 14.73457 16
TACCA 25135 2.1696358 7.695588 7
CTACC 21870 2.1179 8.601697 6
TTTTG 42565 2.0945175 5.986103 32
CAGCG 22065 2.0883656 5.164614 12
AAAAT 30085 2.063292 5.949753 6
CAGTT 28390 1.9451834 6.314393 10
AGCAG 22920 1.9336088 14.253418 18
GCTAC 22500 1.9267886 7.577433 5
ATTGG 31365 1.9003578 5.3659377 2
GATTG 30895 1.8718814 5.5429144 1
AATGC 24495 1.869736 12.373215 24
TGGGG 27715 1.8411943 7.1191926 25
GCGGT 24350 1.8293165 13.246874 11
AACGC 19105 1.8226622 5.632788 2
CGGAA 21510 1.8146564 14.158414 4
CATGG 23495 1.7791879 5.355431 3
AAGCT 22980 1.754094 7.1625223 3
TGATT 31760 1.7410829 5.7059565 1
ATGAT 28260 1.7259125 5.2339535 9
CTGGG 22805 1.713247 6.4269476 25
TGCCG 20095 1.7071947 13.993168 26
GTTAA 27825 1.6993457 5.715371 29
CTGCG 19790 1.681283 6.154101 6
GAAGA 22280 1.6754078 12.645331 6
AATGA 24495 1.666601 5.766441 1
GCGTC 19150 1.626911 5.7573137 8
AGCTA 21145 1.6140262 6.635615 4
AAATG 23715 1.6135309 5.7490163 7
GAAGT 23185 1.5649657 5.9145184 26
ATATG 25295 1.544832 5.458144 15
CGGTT 22075 1.500512 11.162655 12
GTCAT 21800 1.49366 5.0500455 1
ATCGG 19515 1.4777975 12.733374 2
GATCG 19170 1.4516721 12.82294 1
TAAAG 21175 1.4407133 6.6422977 4
GCCGA 14955 1.415432 14.769472 27
AACAC 14620 1.4059277 8.280181 32
AAGTT 22550 1.3771877 5.1942677 27
ATGCC 15850 1.3573153 13.734289 25
TTAAC 19520 1.3481293 6.1809945 30
CAGGA 15695 1.3240832 13.596913 20
TTCAG 19285 1.3213409 11.5020895 15
AAGAG 17115 1.2870109 12.344844 7
AGAGC 15075 1.271778 13.747323 8
GAGCG 15080 1.2621149 13.6959 9
TTAAA 20490 1.2613773 5.8720865 3
TAACA 15970 1.2287518 6.5856347 31
TCGGA 15575 1.1794362 12.252182 3
GTTCA 16025 1.0979769 11.20424 14
AGGAA 14155 1.0644253 12.111325 21
GGTTC 14205 0.96556187 10.9279 13
ACTTC 12405 0.96116316 5.2970457 2
GAATG 13695 0.92439973 10.722869 23
CTTAA 13110 0.905429 6.15233 2
CCGAG 9310 0.881155 13.991375 28
ACACT 9790 0.845066 6.9570556 33
GGAAT 12385 0.8359759 10.6369095 22
GCTTA 12045 0.8252814 6.386474 1
CACTT 10520 0.8151097 6.675085 34
ACTTT 11155 0.6915342 5.567733 35
CGAGA 8035 0.6778598 6.4156566 29
Produced by FastQC (version 0.9.5)