FastQC Report![[OK]](Icons/tick.png)
Basic Statistics
| Measure | Value |
|---|---|
| Filename | 454_SRR073599.fastq.gz |
| File type | Conventional base calls |
| Encoding | Sanger / Illumina 1.9 |
| Total Sequences | 125000 |
| Sequence length | 44-2042 |
| %GC | 43 |
![[FAIL]](Icons/error.png)
Per base sequence quality
![[WARN]](Icons/warning.png)
Per sequence quality scores
Per base sequence content
Per base GC content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
| Sequence | Count | Percentage | Possible Source |
|---|---|---|---|
| CTTAGGTAGGCAAATGTAAGACTAAGGTTGGGTCTTGCTGGAGGAGTTTT | 256 | 0.20479999999999998 | No Hit |
Kmer Content
| Sequence | Count | Obs/Exp Overall | Obs/Exp Max | Max Obs/Exp Position |
|---|---|---|---|---|
| CGTAC | 119675 | 2.3381915 | 37.06815 | 700-799 |
| GTACG | 121920 | 2.30756 | 35.90892 | 700-799 |
| TACGT | 148110 | 2.253356 | 33.438534 | 700-799 |
| ACGTA | 152245 | 2.219705 | 33.489307 | 700-799 |
| GGGGG | 73075 | 2.1637483 | 809.3666 | 2000-2037 |
| CCCCC | 61950 | 2.150167 | 112.54394 | 1500-1999 |
| CCCGG | 55325 | 1.8020015 | 46.69456 | 2000-2037 |
| AACCC | 92570 | 1.7891735 | 33.86608 | 2000-2037 |
| CCGGG | 55200 | 1.7417032 | 86.53514 | 2000-2037 |
| GGGTT | 89950 | 1.7209715 | 37.13423 | 2000-2037 |
| AAACC | 115790 | 1.6700475 | 53.517292 | 2000-2037 |
| GGTTT | 106735 | 1.6415218 | 29.397549 | 2000-2037 |
| TTTTT | 164965 | 1.6393294 | 57.65075 | 1500-1999 |
| AAAAA | 199720 | 1.604097 | 68.92862 | 1500-1999 |
| CCCCG | 47430 | 1.5947236 | 50.49731 | 2000-2037 |
| CGGGG | 50615 | 1.5470908 | 275.43796 | 2000-2037 |
| ACCCC | 59395 | 1.5383544 | 42.994034 | 2000-2037 |
| CCGGT | 60065 | 1.5234346 | 15.349491 | 900-999 |
| CCGTT | 74715 | 1.523272 | 14.49737 | 1000-1499 |
| ACGGT | 80390 | 1.5215285 | 12.233916 | 600-699 |
| GTTTT | 122540 | 1.5149035 | 35.4462 | 2000-2037 |
| AAAAC | 139970 | 1.5064974 | 21.216255 | 2000-2037 |
| ACCGT | 76870 | 1.5018741 | 12.5378065 | 600-699 |
| GGGGT | 62830 | 1.4954511 | 93.83621 | 2000-2037 |
| AACGG | 81995 | 1.4872103 | 12.812057 | 1000-1499 |
| ACCGG | 60345 | 1.4667314 | 153.31853 | 2000-2037 |
| ACGTT | 94925 | 1.4441954 | 14.137788 | 600-699 |
| TTTTG | 116610 | 1.4415939 | 5.554071 | 1 |
| ACCCG | 57315 | 1.4380573 | 19.495502 | 1000-1499 |
| CCCGT | 54780 | 1.4342439 | 16.583103 | 1000-1499 |
| CGGGT | 57260 | 1.406874 | 32.2885 | 2000-2037 |
| AACGT | 95960 | 1.39908 | 14.051213 | 600-699 |
| GTTAC | 91135 | 1.386534 | 11.925655 | 600-699 |
| GGTAC | 73240 | 1.3862015 | 7.776051 | 700-799 |
| ACGGG | 58620 | 1.3802462 | 247.53964 | 2000-2037 |
| GTACC | 69715 | 1.3620808 | 7.256813 | 600-699 |
| GTAAC | 92925 | 1.35483 | 11.836176 | 600-699 |
| TTACC | 86160 | 1.3531616 | 8.505197 | 1000-1499 |
| CGGTA | 71135 | 1.3463606 | 10.108196 | 600-699 |
| GGTAA | 94860 | 1.3397901 | 17.133087 | 2000-2037 |
| TAAAC | 119270 | 1.3395462 | 177.11354 | 2000-2037 |
| CCCCT | 48955 | 1.3231121 | 6.152454 | 1500-1999 |
| CGGTT | 66915 | 1.3215836 | 14.532311 | 900-999 |
| AACCG | 70285 | 1.3159707 | 147.63145 | 2000-2037 |
| CCTTT | 78890 | 1.2928821 | 5.759142 | 1500-1999 |
| TACCG | 65935 | 1.2882278 | 9.259949 | 600-699 |
| CCCTT | 61195 | 1.2879063 | 9.833765 | 1500-1999 |
| CCGTA | 65460 | 1.2789472 | 9.742522 | 600-699 |
| GTTTA | 107650 | 1.2753462 | 11.153843 | 2000-2037 |
| TACGG | 67310 | 1.2739654 | 298.47183 | 2000-2037 |
| CGTTA | 82745 | 1.258888 | 14.492296 | 600-699 |
| TTACG | 82500 | 1.2551606 | 479.8446 | 2000-2037 |
| TTTTA | 130640 | 1.2441064 | 25.029686 | 2000-2037 |
| CGTAA | 84810 | 1.2365148 | 12.243851 | 600-699 |
| TAAAA | 147365 | 1.235084 | 18.881191 | 2000-2037 |
| CGTTT | 76465 | 1.2139509 | 12.22359 | 1000-1499 |
| GGGTA | 66145 | 1.2127644 | 23.131016 | 2000-2037 |
| AAGGG | 68835 | 1.2094717 | 17.592646 | 2000-2037 |
| TAACG | 82685 | 1.2055327 | 13.538576 | 700-799 |
| GGTTA | 81540 | 1.2017592 | 9.432807 | 900-999 |
| TACCC | 59370 | 1.1974083 | 9.801348 | 1500-1999 |
| AAAGG | 88325 | 1.1954854 | 13.770642 | 2000-2037 |
| AAACG | 85460 | 1.1940489 | 11.923181 | 900-999 |
| TTTAC | 97415 | 1.1913476 | 7.256476 | 1000-1499 |
| GTAAA | 109195 | 1.188039 | 25.418472 | 2000-2037 |
| TAACC | 78800 | 1.1859792 | 9.120305 | 800-899 |
| AAAAG | 110820 | 1.1554549 | 10.781775 | 2000-2037 |
| TCCCC | 42135 | 1.1387872 | 6.199435 | 1500-1999 |
| TTAAA | 129330 | 1.1310831 | 19.702553 | 2000-2037 |
| AAAAT | 134430 | 1.1266742 | 13.9124565 | 1500-1999 |
| TTTAA | 122215 | 1.1153543 | 16.93147 | 2000-2037 |
| GAAAC | 79510 | 1.1109154 | 5.613644 | 1500-1999 |
| AGGGT | 60290 | 1.1054134 | 18.071108 | 2000-2037 |
| GGGAA | 61845 | 1.0866531 | 7.9166894 | 1500-1999 |
| AATTT | 118900 | 1.085101 | 7.6755996 | 1500-1999 |
| AAATT | 123345 | 1.0787399 | 9.678446 | 1500-1999 |
| GGTCC | 41910 | 1.0629677 | 7.4760795 | 1500-1999 |
| GTTAA | 93325 | 1.059544 | 9.391855 | 900-999 |
| TTAAC | 90275 | 1.0580034 | 8.644595 | 800-899 |
| GTCCC | 39045 | 1.0222719 | 10.0703 | 1500-1999 |
| GGGTC | 37780 | 0.92825174 | 7.5235333 | 1500-1999 |
| TAAGG | 60315 | 0.8518811 | 5.120233 | 1500-1999 |
| ATTTA | 89605 | 0.81775004 | 6.1899996 | 1500-1999 |
| CGAAA | 55250 | 0.7719542 | 6.1204314 | 1500-1999 |
| CGTCT | 32785 | 0.66841304 | 7.1845894 | 900-999 |