Bismark

Bismark Processing Report

lane1_R1.fq.gz

Data processed at 10:23 on 2013-08-16


Alignment

Sequences analysed in total 447299373
Single-end alignments with a unique best hit 253328964
Sequences without alignments under any condition 145567291
Sequences that did not map uniquely 48403118
Genomic sequence context not extractable (edges of chromosomes) 17

Cytosine Methylation

Total C's analysed 5232303260
Methylated C's in CpG context 114439471
Methylated C's in CHG context 47636473
Methylated C's in CHH context 118416606
Unmethylated C's in CpG context 164043167
Unmethylated C's in CHG context 1204069440
Unmethylated C's in CHH context 3583698103
Percentage methylation (CpG context) 41.1%
Percentage methylation (CHG context) 3.8%
Percentage methylation (CHH context) 3.2%

Alignment to Individual Bisulfite Strands

OT 0 original top strand
CTOT 120197022 complementary to original top strand
CTOB 133131942 complementary to original bottom strand
OB 0 original bottom strand

Deduplication

Alignments analysed 253328947
Unique alignments 101795844
Duplicates removed 151533103
Duplicated alignments were found at 54946655 different positions

Cytosine Methylation after Extraction

Total C's analysed 2044785808
Methylated C's in CpG context 45906670
Methylated C's in CHG context 15967306
Methylated C's in CHH context 44686080
Unmethylated C's in CpG context 52383416
Unmethylated C's in CHG context 461876522
Unmethylated C's in CHH context 1423965814
Percentage methylation (CpG context) 46.7%
Percentage methylation (CHG context) 3.3%
Percentage methylation (CHH context) 3.0%

M-Bias Plot